Determination of Five Common Veterinary Anesthetics in Whole Blood by Gas Chromatography-triple Quadrupole Mass Spectrometry
An analytical method was established for determination of 5 common veterinary anesthetics (tiletamine, xylazole, ketamine, xylazine and zolazepam) in whole blood by using programmed temperature vaporizer (PTV) system combined GC-MS/MS. Those samples were dealt with salting-out-assisted liquid-liquid extraction (SALLE), introducing NaCl as salting-out regent and acetonitrile as extraction solvent. After that, the extracting solution was purified by QuEChERS nano column. Most acetonitrile in the solution were removed by PTV system before analyzing in GC-MS/MS for protection of gas chromatogra
